Hmm .. this is really sparse information, is once again World Savings Day
Sorry, but "Latest" firmware is no valid version .. please use explicit version numbers, because a) you might be wrong .. b) with the release of a new version this information is wrong and makes support harder or confuses ppl when reading it later.
Few questions come to my mind to get an overview about your setup
What computer (HW, e.g. CPU, mainboard, chipset) ?
What OS and version ?
Did you install the RME ASIO driver, if yes which version ?
Do you see USB errors in the driver settings ?
Did you try all other USB2/USB3 ports on your computer ?
Did you try another USB cable ? How long is your USB cable ?
And in what situation does the problem happen, how do you try to play audio ?
What application do you use for playing sound ?
What driver are you using in the application (Windows Driver or ASIO driver ... ?!)
With Windows you have the choice to either install the ASIO driver or not....
Please describe the exact steps that are needed to reproduce your problem.
BR Ramses - UFX III, 12Mic, XTC, ADI-2 Pro FS R BE, RayDAT, X10SRi-F, E5-1680v4, Win10Pro22H2, Cub13