Check the routing for your Phones Output
1. Check that you are in submix mode, the default routing mode, see light blue area in the upper right.
2. In the bottom row, select the phones output: HW Output AN 3/4.
3. Check according to the fader positions of channels in the
- top row (HW inputs) and
- middle row (SW playbacks = audio from PC)
whether all needed audio channels are being routed into the selected submix.
Check that all needed channels are routed to the selected headphone submix.
You might only need to raise the fader of the Mic input.
Some more general info
RME recording interfaces have a very flexible routing. Each of the HW outputs in the bottom row has an individual routing.
You can check the routing of all submixes / HW outputs in the three steps that I told you; see above.
Save your settings
Save the refined routing to one of the 8 Snapshots.
To save your settings (Snapshots/Routing, Window Size and Position), save the workspace to the computer.
Save also to worspace quick select slot #1 (of 30).
The Workspace Quick Select Slots 1..9 can easily be recalled with the key combination ALT-1 .. ALT-9 (Windows)
Option-1 .. Option-9 (macOS).
Source of information
You should read the manual were everything is explained what you need to know.
Other sources of information see this intro posting
https://forum.rme-audio.de/viewtopic.php?id=34394
There you also find information about RME YouTube videos which explain briefly every topic that you need to know to operate TotalMix FX.
BR Ramses - HDSPe MADI FX, M-1620 Pro D, 12Mic, UFX III, ADI-2 Pro FS R BE, Nuendo 15, Win10 IoT Ent